OSAKA UNIV. CBCMP

OSAKA UNIVERSITY CHEMISTRY - BIOLOGY COMBINED MAJOR PROGRAM

Chemistry Biology Experiments 1

Outline

Chemistry & biology experiments 1 (3 credits) is basically open to the 2nd year students in CBCMP in the 4th semester. It consists of Chemistry part and Biology part. Students are required to take part in both parts to get the credits. Here, only Biology part will be explained. Please refer to another tab ‘Chemistry’ for Chemistry part.

 

Course Content ~Biology part~:

Week 1: Guidance

Week 2: Basic lab techniques (pipetman, pH meter, buffer preparation)

Week 3: Quantitative analysis of proteins

Week 4: Ion-exchanging chromatography

Week 5: SDS-PAGE

Week 6: Developmental biology in Xenopus laevis

Week 7: Immunostaining

Week 8: Protein identification by MALDI-TOF-MS

Each experimental class is timetabled for 5 hours, from 1:00 pm to 6:00 pm.

Learning outcomes

One aim of this course is to provide students with basic skills used in biological research. Another aim is to provide students with an opportunity to learn how to do research. At the end of this course, students should:

  • be familiar with fundamental laboratory equipment used in biological research.
  • have acquired basic skills in handling proteins.
  • be able to analyze and discuss scientific data.
  • be able to develop their ability to write a laboratory report/research paper.

In addition to handling proteins, this course will use a live animal, a frog, to examine the effect of a certain chemical on its embryogenesis.

Students are expected to strengthen their knowledge of biology by hands-on experience. This course is also intended as training for doing graduate research.

Textbook

Chemistry & Biology Experiments 1 ~Biology part ~

(To be provided on the Guidance day)

Grading

Attendance is crucial, and late arrival is subject to score reduction. Active participation in actual experiments is graded based on behavior, preparation for lessons, and laboratory notebook. Reports and exercises submitted after each experiment are also evaluated. Submission of all reports is essential. Late submission is penalized by a deduction of points and lack of submission will result in an “F” in the course.

Active class participation: 50%, Exercises and Reports: 50%.

Instructor

Assistant Professor

Atsuko Yamada

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